1 1 2 Receptor usage and cell entry of porcine epidemic diarrhea coronavirus 3 4

نویسندگان

  • Chang Liu
  • Jian Tang
  • Yuanmei Ma
  • Xueya Liang
  • Yang Yang
  • Guiqing Peng
  • Qianqian Qi
  • Shibo Jiang
  • Jianrong Li
  • Lanying Du
  • Fang Li
چکیده

1 Department of Pharmacology, University of Minnesota Medical School, Minneapolis, 8 MN 55455, USA 9 2 Lindsley F. Kimball Research Institute, New York Blood Center, New York, NY 10065, 10 USA 11 3 Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State 12 University, Columbus, OH 43210, USA 13 4 State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, 14 Huazhong Agricultural University, Wuhan, Hubei, China 15 5 Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, 16 Shanghai Medical College and Institute of Medical Microbiology, Fudan University, 17 Shanghai, China 18 19 20 #These authors contributed equally to this work. 21

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Receptor usage and cell entry of porcine epidemic diarrhea coronavirus.

Porcine epidemic diarrhea coronavirus (PEDV) has significantly damaged America's pork industry. Here we investigate the receptor usage and cell entry of PEDV. PEDV recognizes protein receptor aminopeptidase N from pig and human and sugar coreceptor N-acetylneuraminic acid. Moreover, PEDV infects cells from pig, human, monkey, and bat. These results support the idea of bats as an evolutionary or...

متن کامل

Cell Entry of Porcine Epidemic Diarrhea Coronavirus Is Activated by Lysosomal Proteases.

Porcine epidemic diarrhea coronavirus (PEDV) is currently devastating the United States pork industry by causing an 80-100% fatality rate in infected piglets. Coronavirus spike proteins mediate virus entry into cells, a process that requires the spike proteins to be proteolytically activated. It has been a conundrum which proteases activate PEDV entry. Here we systematically investigated the ro...

متن کامل

Identification and Comparison of Receptor Binding Characteristics of the Spike Protein of Two Porcine Epidemic Diarrhea Virus Strains.

Porcine epidemic diarrhea virus (PEDV), a member of Alphacoronavirus, has caused huge economic losses for the global pork industry recently. The spike (S) protein mediates PEDV entry into host cells. Herein, we investigated the interactions between the S protein and its receptor porcine aminopeptidase N (pAPN) or co-receptor sugars. The C-terminal domain (CTD) of the S1 domain is bound to pAPN....

متن کامل

Identification of two mutation sites in spike and envelope proteins mediating optimal cellular infection of porcine epidemic diarrhea virus from different pathways

Entry of the α-coronavirus porcine epidemic diarrhea virus (PEDV) requires specific proteases to activate spike (S) protein for the membrane fusion of the virion to the host cell following receptor binding. Herein, PEDV isolate 85-7 could proliferate and induce cell-cell fusion in a trypsin independent manner on Vero cells, and eight homologous mutation strains were screened by continuous proli...

متن کامل

Weight of the evidence linking feed to an outbreak of porcine epidemic diarrhea in Canadian swine herds

Porcine epidemic diarrhea (PED) is a highly contagious disease of swine caused by the porcine epidemic diarrhea virus (PEDV), an Alphacoronavirus of the Coronaviridae family.1 Swine enteric coronavirus diseases (SECDs) have been known for decades, but PEDV was reported for the first time in Canada in January 2014, nine months after it was first discovered in the United States in May 2013. Even ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2015